Presentation: 3C03

Different types of kinase assays (e.g. Ser-kinase or Tyr-kinase, primary HTS or selectivity) have different needs and limitations as different assay technologies have their specific strengths and weaknesses. High data quality is crucial for all assays, but throughput and costs are important mainly for HTS assays. For selectivity assays the speed of assay development might be more important. Each assay type can be optimally addressed by a certain assay technology. However, for optimal data comparability it is desirable to use a single assay technology for all kinase assays. Based on our standard kinase assay technology HTRF we have built up a whole platform for the fast and cost-effective development and performance of all kinase assays, primary HTS and compound characterization, in the 5-µL-scale. It comprises tools for the rapid identification of high-quality peptide substrates and suitable corresponding specific antibodies, and standard procedures for effective automated assay development and compound profiling.

Close Window X