Presentation: 4A01

Over the past decade plus, a variety of in vitro assays have been developed for the prediction of ADME/Tox properties. These include, permeability, metabolic stability, solubility, cytochrome P450 inhibition, plasma protein binding, log D, metabolite identification, drug transport and several mechanisms of toxicity. Each of these assays have been developed using a variety of protocols and biological components. Most of these assays offer a considerable increase in assay throughput and reduction in cost relative to in vivo assays. However, most assays also have limitations and caveats which make them more suitable for some chemical series and not others. Moreover, the mechanism of action for the ADME endpoint can switch within a chemical series which can confound data interpretation. The state of the science will be reviewed, caveats and limits discussed and several new technologies profiled.

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