| Comprehensive Gene Expression Profiling of GPCRs and Ion Channels |
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Presentation: 1B04 Session: From Genome to Therapeutic TargetsAndrea Brambilla, Francesca Zolezzi, Paolo Guarnieri, Chiara Villa, Claudio Gattuso and Paola Tarroni, Axxam Presenting Author: Paola Tarroni, Axxam - Italy GPCRs and ion channels represent the most attractive target classes for the drug discovery industry. In order to acquire more knowledge about these important target classes, a large scale expression profiling approach was applied using QPCR. A high quality human tissue collection, containing 50 different tissues mirroring the human body, was established for that purpose. Several software tools, for the analysis and visualization of the obtained data were additionally developed. Currently the expression profiles for all human rhodopsin-like GPCRs and a substantial number of the ion channels were obtained and analyzed. Several expression profiles will be shown with an emphasis on those obtained from: a.) different splice variants of the same gene; b.) human genes and their rat orthologue showing quite substantial species differences; and c.) known targets, which will explain the observed side effects of the corresponding drugs. |