Use of Flow-through Peptide Microarrays in Cell-lysate, Kinase and Inhibitor Screens

Presentation: 3C05

Session: Target Biology & Screening: Kinases & Proteases

Rinie van Beuningen, Dirk D Pijnenburg, Diana D Melchers, Riet R Hilhorst, Piet P Boender, Adrienne A van de Berg and Rob R Ruijtenbeek,
PamGene International BV

Presenting Author: Rinie van Beuningen, PamGene International - The Netherlands

    We present an application overview of kinase activity profiling using a novel real-time mix-and-read microarray system. This allows enzymatic kinase activity and inhibitor profiling studies on both biochemical single-plex kinase assays as well as cell-lysates using flow-through, PamChip® peptide arrays. Each array contains up to 400 peptides containing phophosites. The system allows Ki, IC50 and MOA to be measured in one array.

    Several 96-array microtiterplates can be processed per hour using fully automated, robot compatible, PamStation® array platforms. Each plate can reveal up to 40,000 individual enzymatic reactions. The same system can also be used in gene expression, ADMET and genotyping applications.

    This format is aimed to facilitate and accelerate pivotal steps in the drug discovery process from target validation (e.g. signal transduction pathway studies), to assay development (rapid identification and optimization of specific assay peptide substrates) to secondary screening and ADMET studies (side effect assessment).


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