Presentation: 2C01

Just as small molecules can affect different cell types in unique ways, siRNA's can have variable effects depending on the cell type in which they are being transfected. Therefore, when seeking to identify genes involved in a specific cellular process, multiple cell types should be included in the analysis to ensure that any phenotypes are not missed (false negatives) due to cell type specific responses to the siRNA. We have implemented a technique that enables multiple cell lines to be transfected and assayed within a single microtiter well to assess the cell type specific responses to multiple siRNA's. This industry case study demonstrates the value of profiling and validating siRNA responses across multiple cell lines since we identified siRNA activities that would have been missed if only a single cell type were to be used. This case study provides evidence that a more complete understanding, and more precise validation of targets, can be obtained by assessing siRNA knockdown effects on multiple cell lines.

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