| An Innovative Nanoprobe Technology for High-Throughput/High-Content Discovery of Drugs and Target Proteins in Living Human Cells |
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Presentation: 4C02 Session: Advanced Technology for Drug Discovery - Part 1Jaejoon Won, Mina Kim, Yong-Weon Yi and Tae Kook Kim, Korea Advanced Institute of Science and Technology Presenting Author: Tae Kook Kim, Korea Advanced Institute of Science and Technology - South Korea Screening of drugs and identification of their targets are critical for improving therapeutics. All such developed technologies suffer from diverse problems including erroneous experimental outputs due to the use of an artificial milieu such as in vitro binding conditions or non-human cells. Thus, it is desirable to directly screen and probe the interaction of a small molecule with its target(s) in a physiologically and pharmaceutically relevant context with high sensitivity and selectivity. Here we present a novel technology by which drug screening and target identification, based on the inducible movement of nanoprobes, can be systematically performed in human cells at the single cell level. Our innovative nanotechnology has been validated for probing and visualizing a variety of molecular (e.g. drug-protein and protein-protein) interactions in live cells. Using this technological platform, we have succeeded in screening and identifying both small molecules (drug hits) and their target proteins which specifically affect oncogenic processes. |