| Population Patch Clamp Delivers Faster, Cheaper and Better Data |
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Presentation: 3A02 Session: Target Biology & Screening: Ion ChannelsAlan Finkel, Jim Costantin, Andrew Wittel, Naibo Yang, Shawn Handran, Jan Hughes, Molecular Devices Corporation Presenting Author: Alan Finkel, Molecular Devices - USA It is conventional wisdom that when it comes to improving a technique or designing a new product you should aspire to be faster, cheaper and better, then pick any two. The Population Patch Clamp is a fundamentally new patch clamp technique for screening that defies conventional wisdom and allows for substantially greater throughput at lower cost and better data consistency. A planar population patch clamp will be described in which there are 64 apertures per well. A single amplifier records the ensemble average voltage-clamp current. Success rates above 95% are routinely achieved and the coefficients of variation are three or more times better than from single-cell patch clamps. These two benefits combined eliminate the need for redundant compound applications and allow greater numbers of compounds to be tested per day at lower cost. Kinetic responses and IC50s are consistent with equivalent single-cell patch clamp. |