Presentation: 1B01

We have developed a high throughput technology for functional discovery and validation drug targets associated with specific signaling pathway. A lentiviral siRNA library with siRNA sequences for all known genes and fluorescent transcriptional reporter vector are transduced into target cells and cells with modulation in specific pathway are isolated by FACS. Lentiviral siRNA inserts and corresponding target genes associated with specific pathway are then identified by hybridization with microarrays designed with probe sequences that are complementary to every siRNA. Data will be presented that demonstrates the application of genome-wide functional analysis based on the combined use of siRNA libraries and reporter vectors for the discovery drug targets controlling p53-dependent pathway and apoptosis in several cancer cell models.

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