Presentation: P02013

Angiogenesis affects many diseases including cancer and macular degeneration. Angiogenic assays are often indirect, manual, time consuming, labor intensive, difficult to quantify, and expensive. The availability of a reliable, automated, quantitative functional angiogenic assay would benefit drug discovery for several therapeutic areas. We developed an automated, quantitative, High Content Screening angiogenic micro-capillary tube formation assay. Human endothelial cells were plated with pro- or anti-angiogenic compounds on a Matrigel® layer in a 96-well plate. After a time period in which angiogenic tubes formed in the positive controls, the samples were fixed, fluorescently labeled, automatically imaged on Cellomics’ ArrayScan® HCS Reader, and quantitatively analyzed on-the-fly by the Tube Formation BioApplication software module. Multiple features relevant for angiogenesis and angiogenic micro-capillary tube formation were automatically measured. This automated assay performed robustly and reliably, thus enabling the large-scale, quantitative, screening and characterization of compounds affecting both pro- and anti-angiogenic pathways.

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