Automated Dispensation of Yttrium Oxide SPA Imaging Beads, into 1536-Well Plates using the Deerac Fluidics’ Equator™ HTS - Eight Tip Pipetting System.

Presentation: P03045

Session: Robotic Screening & Automation Technologies - Poster Session

Aoife Gallagher,
Deerac Fluidics,
Mike Looker and Ruth Waythe,
GE Healthcare

Presenting Author: Aoife Gallagher, Deerac Fluidics - Ireland

    The drive towards miniaturization within the pharmaceutical field has created a need for liquid handling technologies that accurately deliver low volume reagents to high-density well plates. GE Healthcare’s scintillation proximity assays provide an innovative approach for assay development and biochemical screening that allows the rapid and sensitive measurement of a wide variety of molecular interactions in a homogeneous system.

    Here we demonstrate the successful combination of the use of the Equator™ HTS eight tip pipetting system to dispense Streptavidin coupled Yttium Oxide (YOx) imaging beads. 2µl of YOx beads were dispensed into1536-well plates containing an excess of 3H-biotin. Plates were read on LEADseeker and analyzed for accuracy and reproducibility. CVs of 6-7% were obtained. Outliers were within spec of 10 or less per 1000 wells.

    These results demonstrate the efficacy of the Equator HTS with inbuilt stirred reservoir for the automation of the dispensation of YOx beads to high density plate formats.


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