| Compound Concentration and the Effect of the Tecan Genesis. Methods used to Minimize Dilution Effects |
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Presentation: P03046 Session: Robotic Screening & Automation Technologies - Poster SessionBrian Lupotsky, Sue Holland, Terrence Johnson, Robert Hughes, Stephen Trowbridge, Darren Rimmer, Trevor Dye, Ken Murray, Zoe Blaxill, Steve Besley, Rob Lifely, Zofia Huckins and Donald Lyerly, GlaxoSmithKline Presenting Author: Brian Lupotsky, GlaxoSmithKline - USA The 8-Span Fixed Tip Tecan Genesis is used extensively in the Compound Management organization at GSK, for the solubilization and subsequent dispensing of compounds. Recently, data from CLND analysis of a group of program compounds highlighted a problem with compound concentration. Experiments identified that DMSO used to wash the tips was causing the compound dilution. The level of dilution varied slightly, commonly between 5 and 10%, from Tecan to Tecan despite identical setups. Studies were carried out to minimize dilution effects by varying multiple parameters within the Tecan Software. Significant reduction was found using a combination of a 20µL/s aspiration speed and a 10% partitioned volume. The partitioned volume is an aliquot of the compound, which is pre-aspirated into the tips prior to dispensing the required amount. This method is not ideal as it wastes valuable compound. GSK is currently investigating new non-contact dispensing technology to replace fixed tip instruments. |