| Optimization of Biomek FX Multidispensing Liquid Handling Parameters |
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Presentation: P03041 Session: Robotic Screening & Automation Technologies - Poster SessionGinger Smith, Katja Remlinger, Jim Liacos, Cathy Finlay and Jimmy Bruner, GlaxoSmithKline Presenting Author: Ginger Smith, GlaxoSmithKline - USA The High Throughput Biology department at GlaxoSmithKline utilizes the Biomek FX as the key liquid handling robot. Our department has several applications that require transfer from one 96 well plate to multiple quadrants of a 384 well plate. The multidispensing operation offers the opportunity to reduce the consumable cost associated with these 96 to 384 well transfers and possibly increase throughput. Like any automated liquid delivery application, multidispensing requires specific manipulation of liquid handling parameters such as air gaps, speeds, and heights. Trying to find the right combination of each setting can be time consuming and tedious. This paper describes two methods - One Factor At A Time (OFAT) and Design of Experiment (DOE) - that were used in determining the optimal pipetting parameters for multidispensing applications run on the Biomek FX liquid handling system in 384 well plates. |