| Image-based High-content Screening of Fatty Acid Uptake |
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Presentation: P02029 Session: Imaging Technologies - Poster SessionRichard Sportsman, Mary David, Neal Gliksman, Joe Bosworth, Linnea Hager and Paula Rickert, Molecular Devices Corporation Presenting Author: Richard Sportsman, Molecular Devices Corporation - USA We demonstrate the screening and analysis of Fatty Acid Uptake (FAU) using a cell-based screening kit and the MetaXpress™ application module Cell Scoring. Long-chain fatty acids and their efficient uptake and distribution are critical to cellular function as fuels to generate ATP and as precursors for the biosynthesis of complex membrane lipids. Since increased fatty acid transport into pancreatic beta cells, cardiac myocytes and adipocytes can lead to diabetes, heart failure and obesity, FAU analysis is of interest for drug discovery. Existing FAU detection methods are time-consuming and incompatible for either high content or high throughput screening. Molecular Devices homogeneous QBT™ Fatty Acid Uptake Assay Kit provides a fast, simple and reliable fluorescence-based assay for the detection of fatty acid uptake in cells expressing fatty acid transporters. The assay is ideally suited for both high-content and high-throughput screening and is demonstrated here using MetaXpress™ image acquisition and analysis software. |