Presentation: P03040

Today's high-throughput screening facilities face increasing demands to generate more information from existing compound libraries. An appealing solution is to perform multiplexed assays during the same cell-based screen. A multiplexed-assay approach allows for the evaluation of multiple parameters from the same sample source. An overall decrease in screening costs and variability are also realized when different assays can be used on the same plate of cells. Here we provide proof-of-principle data by combining Promega's cell-based screening assays in a multiplexed format with a variety of high-throughput instrumentation. Live cell reporter, cell viability, and caspase - 3/7 activity assays were combined in 96, 384, and 1536-well formats. A unique combination and detection of both luminescent and fluorescent chemistries within the same well is also demonstrated. The single-reagent additions to each well, extended signal half-lives and sensitivity of each of these compatible chemistry combinations make them ideal for high-throughput liquid handling and detection.

Close Window X