| Development of a Single Use Plate for Compound Storage-Distribution and Miniaturized Assays |
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Presentation: P03035 Session: Robotic Screening & Automation Technologies - Poster SessionPaul B. Taylor, E. Michael August, Richard D. Belval, Mohammed A. Kashem, Jonathan Lipsky, Eugene Margolin, Scott Morley, Efren D. Romero, Michael S. Routburg, Donna Terenzio, Stephen Young and Carol A. Homon, Boehringer Ingelheim Pharmaceuticals Inc. and Innovative Microplate Presenting Author: Paul Taylor, Boehringer Ingelheim Pharmaceuticals Inc. - USA Miniaturization in HTS has received widespread attention in recent years with the focus being on 384, 1536 and higher densities. In analyzing our process we determined that commercially available low volume 384 plates required a minimum dead volume of >7 µL. A secondary observation was that there were very limited options for running low volume homogenous assays in polypropylene rather than polystyrene. We therefore set out to design a new plate with the primary goal of being able to conserve compound by aspirating 2 µL from a 3 µL source. The plate design was done in collaboration with Innovative Microplate and resulted in well shapes with a round bottom to square top 'trumpet flare'. Corners were eliminated to avoid DMSO wicking and the well top tapered to a 3.2 mm cylinder with dimensions similar to that of a REMP 384 minitube (STBR384). Experiences with the plate using various liquid handlers and comparative assay data will be discussed. |