| Compound transfer to 3456-well assay plates using the Echo™ 550 |
|
Presentation: P03038 Session: Robotic Screening & Automation Technologies - Poster SessionFrédéric Massé, Sébastien Guiral, Shiraz Adam, Stacia Kargman and Christine Brideau, Merck Frosst Centre for Therapeutic Research Presenting Author: Stacia Kargman, Merck Frosst Canada & Co. - Canada Many of the HTS assays at Merck are performed in the 3456-well format to enable rapid screening of the sample collection and to reduce the cost of an HTS campaign. Our laboratory has successfully miniaturized many HTS assays to the 3456 format using Aurora Discovery’s Flying Reagent Dispenser™ (FRD) and the tcPR™ detector. However, we did not have the capability to transfer 5nL of compound solutions to the 3456 assay plate. The Echo 550 was chosen to perform this task. We will show reproducibility, accuracy and precision data of the 5nL acoustic droplet ejection into the 3456-well plates using fluorescent dyes. Results from a panel of compounds tested in a 3456-well enzymatic assay will also be presented and compared to the original 384-well format. |