Presentation: P02001

Reactive Oxygen Species (ROS) and NO (Nitric Oxide) are central in apoptosis transduction pathways, and contribute to the activation of the execution machinery. Since apoptosis is a dynamic process of variable duration, and there is high cell-to-cell variability in apoptosis kinetics within cell populations, there is a need to continuously measure apoptosis parameters, including ROS and NO generation rates, in individual, living cells.

To simultaneously monitor multiple apoptosis signals, including generation rates of ROS and NO, we used the Optical LiveCell® Array (Molecular Cytomics Ltd.) technology. This densely packed array of micron-sized wells, each accommodating an individual cell, enables visualization, manipulation and sequential measurement of multiple functional assays of intact living cells, followed by structural and post-fixation studies on the same cells. By performing sequential measurements of individual living cells undergoing apoptosis, the correlation and temporal relationship between the kinetics of ROS and NO generation and the onset of other apoptotic events were investigated.

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