| G1S Checkpoint Reporting |
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Presentation: P02009 Session: Imaging Technologies - Poster SessionSimon Stubbs, Suzanne Hancock, Hayley Tinkler, Nick Thomas, Paul Michael, Stephen Capper, Michael Kenrick, Iain Bye and Dwayne Dexter, GE Healthcare, Jinming Gu and Ellen Fanning, Vanderbilt University Presenting Author: Suzanne Hancock, GE Healthcare - UK Functional elements from the human helicase B gene and human ubiquitin C promoter have been used to develop a sensor of the G1/S checkpoint; the G1/S cell cycle phase marker (G1/S CCPM). High-throughput sub-cellular imaging combined with cell cycle inhibitors and siRNAs have been used to validate cell lines exhibiting stable expression of the sensor. The sensor provides a sensitive and dynamic phenotypic cellular assay and is a powerful tool for functional analysis of the cell cycle enabling the effects of a broad range of agents upon the cell cycle to be monitored. |