| Comparison of Several Classes of Algorithms for Cytoplasm to Nucleus Translocation |
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Presentation: P02025 Session: Imaging Technologies - Poster SessionIlya Ravkin and Vladimir Temov, Vitra Bioscience, Inc. Presenting Author: Ilya Ravkin, Vitra Bioscience - USA Cellular assays assessed by image analysis provide new type of information, but also present several new sources of variability compared to traditional screening. Understanding of these sources and of the behavior of image analysis algorithms under different conditions is important for obtaining reliable data from imaging assays. We have developed several new and implemented several commonly used measures of cytoplasm to nucleus translocation. The quality of these measures is analyzed with respect to user parameters, magnification, number of analyzed cells, accuracy of focusing, depth of field in confocal and non-confocal systems, plate flatness and plate surface treatment. |