| Improving Quality of Biological Assays: Advantages of Direct nl Compound Dispense |
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Presentation: P03015 Session: Robotic Screening & Automation Technologies - Poster SessionMarina Nelen, Kannan Ramachandren and Matthew Todd, Johnson & Johnson Pharmaceutical Research & Development, L.L.C Presenting Author: Marina Nelen, Johnson & Johnson PRD - USA One of the factors affecting accuracy and reproducibility of bioassays, and HTS in particular, is the variability of compound concentration. The integrity of screening collections as well as poor solubility have been widely discussed in recent publications. A less known problem affecting compound concentration is loss due to adsorption to plastic surfaces. Data will be presented showing that compound adsorption is a general phenomenon observed for peptides and organic molecules of various chemotypes. Compound loss can be greater than 80% depending on plate material, solvent conditions and order of addition. Direct nl dispense of compounds from 100% DMSO stock was found to minimize compound adsorption significantly and is currently implemented for screening and secondary assays. Here, we describe our experience with the Hummingbird, nl dispenser from Genomic Solutions Inc., and discuss its advantages for primary screening and hit/lead profiling. |