Presentation: P02005

ATF-2 is a transcription factor activated in response to inflammatory cytokines, UV irradiation, alkylating compounds, and cellular stressors. Once activated, ATF-2 forms complexes with the Jun/Fos family or other ATF family members, and binds to the cAMP Response Element found in the promoter regions of many genes. This initiates transcriptional responses to both viral and cellular signals, resulting in the translocation of ATF-2 from the cytoplasm to the nucleus in responding cells.

In this study, we evaluated several cell lines and stimulants to see which would be the most robust to implement in a High Content Screening assay for ATF-2 translocation. Additional experiments such as effect of cell density, dose responsiveness, and time courses were performed with responsive cell lines to determine the optimal combination to detect activation of ATF-2. Overall, NIH 3T3 cells treated with anisomycin provided the most robust assay for detecting activation and translocation of ATF-2.

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