Presentation: P03010

We present a label-free optical detection method based on the principle of a Young interferometer capable of reading out microtiterplate-formatted wellplates. This technology allows real-time analysis of biomolecular interactions in simple two-step assays. The system yields a high resolution with respect to surface mass coverage and a low sensitivity towards undesired external influences. The key component the device is a planar waveguide chip. Radiation of a low-coherent light source is coupled into the waveguide chip and guided in two separate branches. While one branch serves as reference, the other branch is used as measurement branch to monitor the bioreaction, e.g. binding of molecules. The resulting mass coverage on the chip causes a local change in the phase velocity of the light in the waveguide and is detected by an interferometric set-up. The presented system combines the easy handling of a MTP with the advantage of label-free detection.

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