Presentation: P03003

Nephelometry is a direct method of measuring light scattered by particles suspended in solution at right angles to the beam, or preferably, at a forward angle. Besides classical applications such as compound solubility testing in drug discovery, monitoring of polymerization reactions, and immunoprecipitation, the described application reveals that laser nephelometry is a reliable technique for long term microbial growth monitoring with great reproducibility.

Comparative studies show that nephelometric assays are clearly superior to turbidimetric assays regarding sensitivity. The key advantage of nephelometry is the ability to detect scattered light, even if the concentration of scattering particles is very low, which is the case during the lag phase and beginning of the log phase. When applying BMG LABTECH’s NEPHELOstar for nephelometric measurements, instead of a traditional transmission reader, this early part of the microbial growth curve can be monitored much more accurately.

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