Presentation: P03009

The state-of-the-art of cell cultivation can on the one hand be described as manual cultivation of cells in a cell culture laboratory containing an incubator for the climate control of the cell culture and hand operated microscopes for the daily glance to decide whether passaging or media exchange is necessary. On the other hand, there are systems on the market that carry out the cell cultivation as a rigid industrial process without any inspection of the cell culture.

The aim of the Fraunhofer alliance 'Live Cell Monitoring' is an automatic cell cultivation system where the whole cell culture procedure is executed including optical monitoring, addition of factors, exchange of media, and passaging. This is only feasible by the optical image acquisition of the cell cultures and user-friendly image analysis software that determines the cell culture condition in order to control the cell culture process. The cell culture process can thereby also be documented and archived. The whole cultivation process takes place under cultivation conditions to leave the cell culture in an optimal environment and to avoid stress because of climate changes.

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