| Implementing a Technologically Integrated Approach to the Evaluation of Kinetic Cell Signalling |
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Presentation: P02016 Session: Imaging Technologies - Poster SessionPeter B. Simpson, Grace K.Y. Chan, Sally-Anne Thompson, Amy Platts, Mary Duncan, Kathy G. Sutton, Fahd Choudhry, Keith A. Wafford, Gillian R. Richards and Vahri Beaumont Merck Sharp & Dohme Presenting Author: Peter Simpson, Merck & Co. - United Kingdom High information content kinetic imaging and electrophysiology systems enable a detailed understanding of complex neuronal responses. We obtain dynamic information on cellular activity by coordinated use of innovative multielectrode array assays and cell imaging technologies. Automated camera-based fluorescence imagers are used to characterize differences between immature and mature neuronal cultures in terms of synchronization of signaling. Synchronized calcium oscillations in mature neuronal cultures, which we measure using e.g. BD PathwayHT, are modified by inhibitors and potentiators of ion channels involved in synaptic events. Multi-electrode array technology (Multi-Channel Systems) enables simultaneous recording of spiking behavior in multiple individual cells within a neuronal network in vitro, allowing us to quantitatively assess the extent of connectivity and the strength of a network. This complements intracellular calcium-based connectivity data obtained on higher throughput imagers. Coordinated implementation of electrophysiology and imaging screening assays produces a rounded package of information on cellular and compound activity. |