| Latent Fluorophores Based on the Trimethyl Lock |
|
Presentation: P02024 Session: Imaging Technologies - Poster SessionLuke Lavis, Sunil Chandran, Kimberly Dickson, and Ronald Raines, University of Wisconsin-Madison Presenting Author: Luke Lavis, University of Wisconsin-Madison - USA Fluorogenic substrates are widely used to measure enzyme catalysis in vitro and in vivo. Many known fluorogenic substrates suffer from poor stability in aqueous solution resulting in high background levels. We have prepared a new class of latent fluorophores based on the 'trimethyl lock' pro-drug strategy. These fluorogenic substrates possess low background fluorescence and superior stability while maintaining reactivity with the enzyme of interest. The modular design of the probe allows specificity for a particular enzyme to be incorporated into the molecule by simple functional group manipulations. We initially applied this concept to rhodamine 110, and have now extended this strategy to other dye classes. These hyperstable substrates should prove useful in many applications, including bioimaging and high-throughput screening. |