| High-Throughput Automation of a Dual Reporter Assay in Low-Volume 384 & 1536-Well Plate Formats using the Deerac Fluidics’ Equator HTS- Eight Tip Pipetting System, Promega’s Chroma-Luc Technology, and BMG LABTECH’s PHERAstar Microplate Reader |
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Presentation: P03008 Session: Robotic Screening & Automation Technologies - Poster SessionRod Haroutunian and Aoife Gallagher, Deerac Fluidics Brad Larson, Tracy Worzella and Michael Bjerke, Promega Corporation Ed Matthews, BMG Labtech Presenting Author: Rod Haroutunian, Deerac Fluidics - Ireland The drive towards miniaturization within the pharmaceutical and biotechnology fields has created a need for liquid handling technologies that accurately deliver low volume reagents to high-density plates. This has also created a need for simple, fully scaleable assays in low volumes. Here we demonstrate the successful combination of both through the use of the Equator™ HTS - Eight Tip Pipetting System, and the dual color Chroma-Luc™ technology. Cellular lysates, containing the green CBG99luc and red CBRluc genes, followed by Chroma-Glo™ reagent, were dispensed in low-volume 384, and 1536-well formats using volumes ranging from 10µl to 500nl. Luminescence from the two luciferases was then simultaneously measured using the BMG LABTECH PHERAstar plate reader. The exceptional Z' Factor scores, linearity, limit of detection, and separation of signal data, show the flexibility and reliability of the Equator™ HTS, and Chroma-Luc™ dual reporter technology in any high-throughput situation. |