| Screening of Biologically Active Marine Natural Products in PIBOC |
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Presentation: P02019 Session: Imaging Technologies - Poster SessionDmitry Aminin, Michael Anisimov, Nina Prokof'eva, Irina Agafonova, Galina Likhatskaya and Valentin Stonik, Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences Presenting Author: Dmitry Aminin, Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences - Russia The search for biologically active compounds in the Laboratory of Bioassay of PIBOC consists mainly of cell based screening, image technology and ion channels as an cellular targets as well as several in vivo models. This work includes application of the technique of radioisotopes, ion-selective electrodes, fluorescent spectroscopy, cytofluorimetry, BLM technique, cell image analysis and in silico molecular modeling and docking approach which afford evaluation of interaction of substances with various types of cells, biological and model lipid membranes. We perform our investigations using some cell cultures, embryos of marine invertebrates (Ehinodermata), different microorganisms including two-hybrid transgenic yeasts with human estrogen receptors, and lipid liposomes. We screen natural products for antimicrobial, antiviral and anticancer activity, immunomodulatory effects, as inhibitors of cell adhesion and antioxidants. High resolution MRI-tomography with 7T magnet (PharmaScan 70/16US, Bruker) allows us to search in situ for compounds with anticancer, anti stroke and anti ischemia activity, inhibitors of angiogenesis and hepatoprotective substances. |